Thermo Fisher Scientific WNT10A Polyclonal Antibody
WNT10A 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human 및 Mouse 시료에 반응합니다. Western blot, IHC, ICC/IF에 사용 가능하며, 46 kDa 밴드를 검출합니다. Affinity chromatography로 정제되었으며, PBS/BSA/glycerol buffer에 보관됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:600 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human Wnt10a aa 201–417/417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3093528 |
Product Specific Information
- Positive control: RAW264.7 cell lysates, A375, human kidney tissue, mouse kidney tissue
- Predicted band size: 46 kDa
- Subcellular Location: Extracellular matrix, Secreted
Target Information
The WNT gene family encodes secreted signaling proteins involved in oncogenesis and developmental processes such as cell fate regulation and embryonic patterning.
WNT10A is strongly expressed in promyelocytic leukemia and Burkitt’s lymphoma cell lines.
It is coexpressed with WNT6 in colorectal cancer cell lines, suggesting a role in carcinogenesis via activation of the WNT–β-catenin–TCF signaling pathway.
Both WNT10A and WNT6 genes are clustered in the chromosome 2q35 region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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