Thermo Fisher Scientific Phospho-53BP1 (Ser25) Polyclonal Antibody
Phospho-53BP1 (Ser25) 특이적 폴리클로날 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. Rabbit IgG 기반으로 제조되었으며, 액상 형태로 4°C에서 보관합니다. DNA 손상 반응 및 종양 억제 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phosphorylated synthetic peptide representing a portion of human p53 Binding Protein 1 around serine 25 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the sites of double-strand breaks. It may participate in checkpoint signaling during mitosis, enhance TP53-mediated transcriptional activation, and contribute to DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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