Thermo Fisher Scientific Human PIK3CD/PIK3R1 (p110 delta/p85 alpha), His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

84.7-120.6 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM sodium phosphate, pH 7, with 25% glycerol, 0.1mM PMSF, 0.25mM DTT, 300mM NaCl, 150mM Imidazole

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

PIK3CD/PIK3R1 (p110-delta/p85-alpha) recombinant human protein is:
• Available in 3 convenient sizes
• Validated in our Adapta™ and Kinase Binding assays
• A full length protein that can help advance your research when used with validated assays

Related assay platforms:
LanthaScreen™ Kinase Binding Assay
Adapta™ Kinase Activity Assay

Protein Form: Recombinant, Full Length

The gene accession number for PIK3CD (p110δ) is NP_005017.3 and for PIK3R1 (p85α) is NP_852664.1.

Target Information

Phosphatidylinositol 3-kinase (PI3K) is composed of a catalytic subunit (p110-delta) and a regulatory subunit (p85-alpha in this case), which are encoded by the PIK3CD and PIK3R1 genes. PI3K phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It plays a role in oncogenic transformation and is consistently expressed at a high level in AML blast cells. PI3K can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that leads to neutrophil polarization, and regulates directional migration, but not random locomotion. The PI3K signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.