Thermo Fisher Scientific beta Actin Polyclonal Antibody, Biotin
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Goat, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a C-terminal region near amino acids 350–375 of human beta actin |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against human beta actin protein.
BLAST analysis suggests reactivity with beta actin from a wide range of organisms, including most vertebrates and some yeast.
Broad reactivity makes this antibody an excellent loading control.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein present in all human cell types and is involved in cell motility, structure, and integrity.
Beta and gamma actins coexist in most cell types as cytoskeletal components.
Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) with a double-helical structure.
Because beta actin is ubiquitously expressed in all eukaryotic cells, it is widely used as a loading control in protein detection assays such as Western blotting.
Antibodies to beta actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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