Thermo Fisher Scientific Myc Tag Polyclonal Antibody
Myc 태그 단백질 검출용 염소 폴리클로날 항체로, Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. 합성 펩타이드 면역원으로 제작되어 높은 특이성과 재현성을 제공. 연구용으로 세포 내 Myc 발현 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide representing amino acid residues 410–419 (EQKLISEEDL) of human myc conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with a synthetic peptide representing amino acid residues 410–419 (EQKLISEEDL) of human myc conjugated to KLH.
The antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
Myc is a proto-oncogene encoding a nuclear phosphoprotein involved in cell cycle progression, apoptosis, and cellular transformation.
The protein forms a heterodimer with the transcription factor MAX, binding to the E-box DNA consensus sequence to regulate transcription of specific target genes.
Gene amplification is frequently observed in various human cancers, and translocations are associated with Burkitt lymphoma and multiple myeloma.
Translation may initiate from both an upstream non-AUG (CUG) and a downstream AUG start site, producing two isoforms with distinct N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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