Thermo Fisher Scientific EGF Monoclonal Antibody (OTI2F9), TrueMAB
EGF 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 동결건조 형태로 제공되며, 재구성 시 1 mg/mL 농도로 사용 가능합니다. 인간 EGF 단백질(aa 1054-1207) 기반으로 제작되었습니다. 연구용 전용 제품입니다.
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Applications
Western Blot (WB)
- Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
- Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1054–1207 of human EGF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, to initiate various cellular signaling pathways. EGFR dimerization (homo- or heterodimers) activates downstream cascades such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways. EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. Dysregulation of EGF signaling is associated with several cancers and genetic disorders such as hypomagnesemia type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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