Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), PE, eBioscience
Mouse CD8a 인식 단클론 항체(53-6.7)로 PE 형광체가 결합된 제품. Flow cytometry에 최적화되어 있으며, 0.25 µg/test로 사용 가능. CD8a는 T 세포 발달 및 활성화에 중요한 표면 수용체로, 면역세포 분석 연구용으로 적합.
- 카탈로그번호
- 12-0081-8x (4개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | 358 publications |
| Functional Assay (Functional) | - | 3 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465529 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436–780
- Custom conjugation available upon request
Product Specific Information
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer.
CD8 is expressed on thymocytes, mature αβ T cells, γδ T cells, intestinal intraepithelial lymphocytes, and dendritic cells.
It binds to MHC class I and associates with tyrosine kinase p56lck, playing a key role in T cell development and activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse thymocytes and splenocytes, ≤0.25 µg/test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Antibody titration recommended for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family.
It functions as a co-receptor for MHC class I molecules, aiding T cell receptor (TCR) signaling and cytotoxic T lymphocyte (CTL) activation.
CD8 exists as disulfide-linked heterodimers (CD8αβ) or homodimers (CD8αα).
Upon MHC-I/peptide complex ligation, CD8 recruits Lck kinase, inducing lymphokine production, motility, and CTL activation.
Naive CD8⁺ T cell differentiation into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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