Thermo Fisher Scientific MYOD Polyclonal Antibody
인간 MYOD1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IP에 적합. 보존제가 없는 액상 형태이며, Protein A 및 항원 친화 크로마토그래피로 정제됨. 단기 4°C, 장기 -20°C 보관 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunoprecipitation (IP) | 1–2 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human MYOD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788729 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human MYOD1.
Target Information
The Myogenic determination gene (MyoD) was first identified by its ability to convert embryonic mouse fibroblast cells into muscle cells. Forced expression of the MyoD (human homolog is myf3) gene in various normal and neoplastic cells can either convert the cells to muscle cells or activate otherwise inactive muscle-specific genes.
The regulatory domain of the MyoD gene product lies within a 70 amino acid region comprising a basic DNA-binding motif and a helix-loop-helix (HLH) dimerization motif.
Subsequent studies identified three other genes sharing sequence homology with the basic HLH domain of MyoD: myf5, myogenin (human homolog is myf4), and myf6 (also known as MRF4 and herculin).
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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