Thermo Fisher Scientific LDHA Monoclonal Antibody (OTI2C11), TrueMAB
LDHA 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, IHC, IP에 적합. 인간 반응성, 비결합형, 동결건조 형태. 고순도 친화 크로마토그래피 정제, 연구용으로만 사용.
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Thermo Fisher Scientific LDHA Monoclonal Antibody (OTI2C11), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C11 |
| Immunogen | Full length human recombinant protein of human LDHA produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Lactate dehydrogenase (LDH) catalyzes the conversion of pyruvate to lactate with interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes based on M and H chain proportions in the tetrameric structure.
LDHA expression is hypoxia-inducible and regulated by HIF1a activity. LDHB is often upregulated in cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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