Thermo Fisher Scientific HSPA9 Monoclonal Antibody (OTI9F8), TrueMAB
HSPA9 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 적합합니다. 동결건조 형태로 제공되며 PBS 버퍼에 8% 트레할로스가 포함되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9F8 |
| Immunogen | Full length human recombinant protein of human HSPA9 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The HSP70 family includes four highly conserved proteins: HSP70, HSC70, GRP75, and GRP78.
GRP75 (also known as HSPA9) is localized in the mitochondrial matrix and facilitates the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin.
GRP75 and GRP78 are not induced by heat stress but are upregulated under glucose deprivation conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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