Thermo Fisher Scientific IL-8 (CXCL8) Monoclonal Antibody (3IL8-H10)

Thermo Fisher Scientific IL-8 (CXCL8) Monoclonal Antibody (3IL8-H10)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• M801 targets IL-8 in ELISA and WB applications and shows reactivity with human samples.
• The immunogen is recombinant human IL-8, detecting IL-8 (~9 kDa).
• The M801 IL-8 antibody (clone 3IL8-H10) can be paired in sandwich ELISA with detection antibody M802B (biotinylated conjugate of clone I8-S2).
  • Typical dilutions for sandwich ELISA:
    • Coating: 1 µg/mL
    • Detection: 0.125–0.25 µg/mL
• M801 and biotinylated M802B have been used with recombinant IL-8 protein SIL8 in ELISA applications.
• Endotoxin tested by LAL assay: ≤10 EU/mg.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory chemokine belonging to the CXC subfamily. It binds to receptors IL-8RA and IL-8RB, functioning as a chemoattractant and angiogenic factor. IL-8 expression can be induced by inflammatory stimuli in various cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is stored in Weibel-Palade bodies.

IL-8 contains the ELR motif (Glu-Leu-Arg) and signals through CXCR1 and CXCR2 receptors. Previous names include NAP-1, GCP-1, MONAP, and protein 3-10C. IL-8 and related CXC chemokines form a gene cluster on chromosome 4q. IL-8 is implicated in tumor angiogenesis and growth, and in bronchiolitis pathogenesis caused by viral infection.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.