
Thermo Fisher Scientific EPAS1 Monoclonal Antibody (OTI2G5), TrueMAB
인간 EPAS1 단백질(aa 584-870)에 특이적인 단클론 항체로, Western blot에 최적화됨. 동결건조 형태로 제공되며 PBS(8% 트레할로스) 완충액에 보관. 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
- Western Blot (WB): 1:2,000 dilution tested
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 584–870 of human EPAS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
HIF-2 alpha (EPAS1) is a transcription factor involved in oxygen-regulated gene expression. It contains a basic-helix-loop-helix domain and participates in signal transduction pathways responsive to oxygen levels. HIF-2 alpha regulates vascular endothelial growth factor (VEGF) expression and is implicated in blood vessel and lung tubular system development. Mutations in this gene are associated with familial erythrocytosis type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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