Thermo Fisher Scientific EPAS1 Monoclonal Antibody (OTI2A6), TrueMAB
인간 EPAS1(HIF-2α)에 특이적인 단일클론 항체로, Western blot과 IHC(P)에서 검증됨. 무보존제 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 산소 반응 유전자 연구 및 혈관 발달 관련 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 584–870 of human EPAS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of oxygen-regulated genes.
It contains a basic-helix-loop-helix domain and a dimerization domain, functioning in oxygen-sensing signal transduction pathways.
HIF-2α regulates VEGF expression and contributes to blood vessel development and lung tubular system formation.
Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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