Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Yellow 610

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Request custom conjugation

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Ly108 is also known as NTB-A and is expressed on NK, T, and B cells. Engagement of Ly108 enhances NK cell activity and IFN-gamma expression, and plays a role in X-linked lymphoproliferative disease.

Staining shows no strain differences between C57BL/6 and BALB/c mice. Each product contains one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes and thymocytes at ≤ 0.8 µg/test (100 µL volume, 10⁵–10⁸ cells/test). Antibody titration recommended.

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which improves staining performance. Add 5 µL per sample to antibody cocktails before combining with cells. For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates use Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescence with minimal spillover.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Internal testing shows NovaFluor Yellow 610 may non-specifically stain B cells in SJL mice; not observed in BALB/c or C57BL/6 strains.

Target Information

Ly108 is the mouse homologue of human SLAMF6, expressed on NK, B, and T cells. Activation enhances NK cell activity and IFN-gamma expression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지