Thermo Fisher Scientific CD309 (FLK1) Monoclonal Antibody (Avas12a1), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• Biotin
• PE
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The Avas12a1 monoclonal antibody reacts with mouse Flk-1 (VEGFR2), a receptor tyrosine kinase involved in vascular endothelial tissue development. It is expressed on endothelial cells during embryonic stages and in some adult endothelial tissues.

Applications Reported: Flow cytometry, Western blot, and immunohistochemistry.
Applications Tested: Tested by flow cytometric analysis of bEnd.3 cells. Recommended ≤0.5 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Optimize antibody titration for best results.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase that binds vascular endothelial growth factor (VEGF) with high affinity. It is expressed early in endothelial cell precursors and plays a key role in angiogenesis. The receptor’s signaling and trafficking are regulated by multiple factors such as Rab GTPase, P2Y purine nucleotide receptor, integrin αVβ3, and T-cell protein tyrosine phosphatase. Mutations in FLK1 are associated with infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.