Thermo Fisher Scientific CD34 (soluble) Polyclonal Antibody, PeproTech
Human sCD34 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 줄기세포 표지자인 CD34 검출 연구용으로 활용됩니다.
- 카탈로그번호
- 500-P327-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD34 (soluble) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 27.4 kDa Recombinant Human sCD34 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930434 |
Product Specific Information
AA Sequence of recombinant protein:
LDNNGTATPE LPTQGTFSNV STNVSYQETT TPSTLGSTSL HPVSQHGNEA TTNITETTVK FTSTSVITSV YGNTNSSVQS QTSVISTVFT TPANVSTPET TLKPSLSPGN VSDLSTTSTS LATSPTKPYT SSSPILSDIK AEIKCSGIRE VKLTQGICLE QNKTSSCAEF KKDRGEGLAR VLCGEEQADA DAGAQVCSLL LAQSEVRPQC LLLVLANRTE ISSKLQLMKK HQSDLKKLGI LDFTEQDVAS HQSYSQKT.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sCD34. Anti-Human sCD34-specific antibody was purified by affinity chromatography employing an immobilized Human sCD34 matrix.
Sandwich ELISA:
To detect Human sCD34 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD34 (500-P327) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human sCD34.
Western Blot:
To detect Human sCD34 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human sCD34 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) present on many stem cell populations. It serves as a stem cell marker, although its expression on human hematopoietic stem cells is reversible. CD34 may act as a receptor mediating endocytosis and regulating adhesion, differentiation, and proliferation of hematopoietic stem cells and progenitors. It likely plays a role in stem cell attachment to the bone marrow extracellular matrix or stromal cells and may act as a scaffold for glycans binding to lectins. The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction.
Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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