Thermo Fisher Scientific CD27 Recombinant Rabbit Monoclonal Antibody (034)

Applications

• ELISA (ELISA)
  Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

This antibody has specificity for Mouse CD27.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, which includes CD40 and CD30. Members of this family regulate cell proliferation and death. CD27 and its ligand CD70 are predominantly expressed on lymphocytes.

High expression levels of CD27 depend on antigen receptor ligation. CD70 expression requires additional co-stimulatory or pro-inflammatory signals. CD27 is a disulfide-linked homodimer expressed on mature thymocytes, peripheral blood T cells, and a subpopulation of B cells.

Activation of T cells via the TCR-CD3 complex upregulates CD27 expression and releases soluble CD27 (sCD27), a 28–32 kDa form detected in plasma, urine, or spinal fluid. Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies.

RgpA, a cysteine proteinase, can degrade CD27 and counteract T cell activation mediated by CD27 and CD70. CD27-binding protein (SIVA), a proapoptotic protein, binds to CD27 and plays a role in CD27-mediated apoptosis.

Diseases associated with CD27 dysfunction include:

• Lymphoproliferative Syndrome 2
• Autosomal Recessive Lymphoproliferative Syndrome

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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