Thermo Fisher Scientific Phospho-C/EBP alpha (Thr226) Polyclonal Antibody
인산화된 C/EBP alpha (Thr226)을 특이적으로 인식하는 토끼 폴리클로날 항체. Western blot에 적합하며, 인간, 마우스, 랫트 시료에 반응. 항원 친화 크로마토그래피로 정제되었고, 고순도(>95%) 확보. 연구용으로 단백질 인산화 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human C/EBP alpha around the phosphorylation site of Threonine 226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553735 |
Product Specific Information
- Detects endogenous protein at molecular weights of approximately 30 and 45 kDa.
- Purity >95% by SDS-PAGE.
Target Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors—alpha, beta, delta, and epsilon—are widely expressed in various tissues. These proteins contain an evolutionarily conserved five-amino acid motif (RDM: (I/V/L)KXEP), which plays a role in metabolic regulation in liver and adipose tissue.
C/EBP transcription factors function in cascades that facilitate adipocyte differentiation. Multiple C/EBP beta isoforms (LAP*, LAP, LIP) arise from alternative translation initiation and have distinct roles in gene regulation.
In the nervous system, C/EBPs are involved in nerve growth factor mediation, synaptic plasticity, and inflammatory responses associated with Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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