Thermo Fisher Scientific Phospho-CK1 alpha (Thr321) Polyclonal Antibody
Western blot 및 IHC(P)에서 사용 가능한 Rabbit Polyclonal 항체로, CK1 alpha의 Thr321 인산화 부위를 인식합니다. Human, Mouse, Rat 반응성이 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로 4°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human Casein Kinase I alpha around the phosphorylation site of Threonine 321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553736 |
Product Specific Information
- Detects endogenous CK1 alpha protein (~42 kDa).
- Purity >95% by SDS-PAGE.
Target Information
CNSK1A1 (CK1 alpha 1) is a member of the casein kinase I (CKI) gene family encoding serine/threonine kinases that preferentially phosphorylate acidic substrates. CKI proteins are monomeric (25–55 kDa) and localized in nuclei, cytoplasm, and membrane fractions of eukaryotic cells.
It can phosphorylate multiple proteins and participates in Wnt signaling.
Phosphorylates CTNNB1 at Ser-45 and may also phosphorylate PER1 and PER2.
May play a role in chromosome segregation during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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