Thermo Fisher Scientific 14-3-3 gamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human 14-3-3 gamma (NP_0366112) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:14-3-3 gamma,uniProtId:P61981-1,ncbiNodeId:9606,antigenRange:100-200,antigenLength:247,antigenImageFileName:PA5-91905_14-3-3_gamma_P61981-1_Rabbit.svg,antigenImageFileNamePDP:PA5-91905_14-3-3_gamma_P61981-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.54 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806322

Product Specific Information

Positive Samples: U-251MG, K-562, HeLa, Mouse brain, Mouse heart; Cellular Location: Cytoplasm

Immunogen sequence: VLSLLDNYLI KNCSETQYES KVFYLKMKGD YYRYLAEVAT GEKRATVVES SEKAYSEAHE ISKEHMQPTH PIRLGLALNY SVFYYEIQNA PEQACHLAKT A

Target Information

14-3-3 gamma encodes a product that belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.