Thermo Fisher Scientific IDH3A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_0055211) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IDH3A,uniProtId:P50213-1,ncbiNodeId:9606,antigenRange:28-366,antigenLength:366,antigenImageFileName:PA5-89963_IDH3A_P50213-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89963_IDH3A_P50213-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805868

Product Specific Information

Immunogen sequence: TGGVQTVTLI PGDGIGPEIS AAVMKIFDAA KAPIQWEERN VTAIQGPGGK WMIPSEAKES MDKNKMGLKG PLKTPIAAGH PSMNLLLRKT FDLYANVRPC VSIEGYKTPY TDVNIVTIRE NTEGEYSGIE HVIVDGVVQS IKLITEGASK RIAEFAFEYA RNNHRSNVTA VHKANIMRMS DGLFLQKCRE VAESCKDIKF NEMYLDTVCL NMVQDPSQFD VLVMPNLYGD ILSDLCAGLI GGLGVTPSGN IGANGVAIFE SVHGTAPDIA GKDMANPTAL LLSAVMMLRH MGLFDHAARI EAACFATIKD GKSLTKDLGG NAKCSDFTEE ICRRVKDLD; Positive Samples: Raji, NIH/3T3, Mouse brain, Mouse heart, Mouse kidney; Cellular Location: Mitochondrion

Target Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.