Thermo Fisher Scientific MDC Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MDC (CCL22). Anti-Human MDC (CCL22)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human MDC (CCL22) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MDC (CCL22) (500-P107) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human MDC (CCL22).

Western Blot:
To detect hMDC by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant hMDC is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P107BT-1MG is provided as 2 × 500 µg.

Target Information

CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology.
It is chemotactic for monocytes, dendritic cells, and natural killer cells.
CCL22 is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemotactic activity for neutrophils, eosinophils, and resting T-lymphocytes.
Binds to CCR4.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.