Thermo Fisher Scientific Phospho-Cdc20 (Thr70) Polyclonal Antibody
Phospho-Cdc20 (Thr70) 항체로, 인산화된 Cdc20 단백질을 특이적으로 검출. Western blot에 최적화되어 있으며, Human, Mouse, Rat 시료에 반응. Rabbit polyclonal IgG로 제조되어 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC20 (Accession Q12834), corresponding to amino acid residues around phosphorylated Thr70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817132 |
Product Specific Information
This antibody detects endogenous levels of CDC20 only when phosphorylated at Thr70.
Target Information
CDC20 is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. It is regulated by MAD2L1: in metaphase, the MAD2L1-CDC20-APC/C ternary complex is inactive, while in anaphase, the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates synaptic vesicle clustering at the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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