Thermo Fisher Scientific Agrin Monoclonal Antibody (Agr 33)
Agrin 단백질 검출용 Agr 33 클론 단일클론 항체로, Mouse 및 Rat 시료에 반응. Western blot, IHC, ICC/IF 등 다양한 응용 가능. Protein G 정제, PBS 기반 보관용액, 장기보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Agr 33 |
| Immunogen | Recombinant rat agrin, C-terminal construct |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2, with 0.1mM PMSF, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_557527 |
Product Specific Information
MA1-20378 detects agrin in mouse and rat samples.
Target Information
Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Agrin is responsible for clustering acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants arise from alternative mRNA splicing. Splice forms with inserts at sites designated y and z in the carboxy terminus display high affinity for AChRs, while those lacking these inserts associate weakly. Muscle alpha-dystroglycan has been proposed as the receptor for Agrin clustering activity, though this remains debated. Tyrosine phosphorylation is implicated as an early step in AChR aggregation. A receptor tyrosine kinase, MuSK, interacts with Agrin and is specifically localized to developing muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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