Thermo Fisher Scientific H3K9acS10ph Monoclonal Antibody (APH3-64)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Amphibian, Bovine, C. elegans, Chicken, Fruit fly, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

APH3-64

Immunogen

Synthetic, acetylated and phosphorylated (acetyl K9) & (phospho S10) peptide, conjugated to KLH, corresponding to the N-terminal residues 7-20 of huma if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K9acS10ph,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:7-20,antigenLength:136,antigenImageFileName:MA1-25461_H3K9acS10ph_P68431-1_House_mouse.svg,antigenImageFileNamePDP:MA1-25461_H3K9acS10ph_P68431-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_795745

Product Specific Information

Recommended positive controls: Jurkat treated with Nocodazole.

MA1-25461 is expected to cross react with mouse, rat, Xenopus laevis, bovine, C. elegans, chicken, Drosophilia melanogaster, and a wide range of other species due to sequence homolog

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.