Thermo Fisher Scientific RFC5 Polyclonal Antibody
Thermo Fisher Scientific의 RFC5 Polyclonal Antibody는 인간 및 생쥐 시료에서 RFC5 단백질을 검출하는 고품질 항체입니다. Western blot과 IHC에 최적화되어 있으며, 친화 크로마토그래피로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RFC5 (Accession P40937), corresponding to amino acid residues L71–L121 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852830 |
Product Specific Information
Antibody detects endogenous levels of total RFC5.
Target Information
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires accessory proteins such as proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also known as activator 1, is a protein complex composed of five distinct subunits (140, 40, 38, 37, and 36 kDa). The RFC5 gene encodes the 36 kDa subunit, which interacts with the C-terminal region of PCNA and forms a core complex with the 38 and 40 kDa subunits. This core complex exhibits DNA-dependent ATPase activity stimulated by PCNA in vitro. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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