Thermo Fisher Scientific CD135 (Flt3) Monoclonal Antibody (BV10A4H2), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.5 µg)/test
  View 3 publications

Product Specifications

Product Specific Information

Description:
The BV10A4H2 monoclonal antibody reacts with human CD135 (also known as Flk2 or Flt3), a 135–150 kDa tyrosine kinase receptor expressed on multipotential progenitor cells, including primitive B cell and myelomonocytic progenitors in fetal liver and adult bone marrow.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of REH cell line. Recommended use: 5 µL (0.5 µg) per test in a final 100 µL volume. Cell number should be determined empirically (10⁵–10⁸ cells/test).

Fluorescence Characteristics:
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FLT3 is a tyrosine protein kinase belonging to the growth factor receptor tyrosine kinase family. Activation occurs through ligand binding, inducing receptor dimerization and autophosphorylation. Activated FLT3 phosphorylates downstream effectors involved in apoptosis, proliferation, and differentiation of hematopoietic cells. Constitutive activation mutations are associated with acute myeloid and lymphoblastic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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