Thermo Fisher Scientific CD135 (Flt3) Monoclonal Antibody (BV10A4H2), PE, eBioscience
Human CD135(Flt3) 인식 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. PE 형광체 결합으로 높은 감도 제공. BV10A4H2 클론, IgG1 아이소타입. 4°C에서 보관, 동결 금지. 연구용으로 적합.
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Applications
- Flow Cytometry (Flow)
Tested dilution: 5 µL (0.5 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BV10A4H2 |
| Immunogen | BV-173 leukemic cell line |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10596974 |
Product Specific Information
Description:
The BV10A4H2 monoclonal antibody reacts with human CD135 (also known as Flk2 or Flt3), a 135–150 kDa tyrosine kinase receptor expressed on multipotential progenitor cells, including primitive B cell and myelomonocytic progenitors in fetal liver and adult bone marrow.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of REH cell line. Recommended use: 5 µL (0.5 µg) per test in a final 100 µL volume. Cell number should be determined empirically (10⁵–10⁸ cells/test).
Fluorescence Characteristics:
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FLT3 is a tyrosine protein kinase belonging to the growth factor receptor tyrosine kinase family. Activation occurs through ligand binding, inducing receptor dimerization and autophosphorylation. Activated FLT3 phosphorylates downstream effectors involved in apoptosis, proliferation, and differentiation of hematopoietic cells. Constitutive activation mutations are associated with acute myeloid and lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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