Thermo Fisher Scientific CD133 (Prominin-1) Monoclonal Antibody (TMP4), PE, eBioscience
인간 CD133 (Prominin-1)을 인식하는 TMP4 단클론 항체로, 줄기세포 및 암 줄기세포 마커 연구에 적합합니다. PE 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 4°C 어두운 곳에서 보관합니다.
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- 12-1338-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD133 (Prominin-1) Monoclonal Antibody (TMP4), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | 14 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TMP4 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1582258 |
Additional Formats
- Alexa Fluor 488
- APC
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available
Product Specific Information
The TMP4 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, including Prominin-2. These proteins are expressed in plasma membrane protrusions such as epithelial microvilli. CD133 exists in alternatively spliced isoforms and is glycosylated in a tissue-dependent manner. It was first identified on CD34+ hematopoietic progenitors and is widely used as a marker for stem cells and cancer stem cells, including those from colon, brain, and prostate cancers.
The TMP4 antibody recognizes a distinct epitope from the EMK08 clone, indicating non-overlapping binding sites.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells. Recommended usage is 5 µL (0.25 µg) per test in 100 µL final volume.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD133 (AC133) is a 97 kDa glycoprotein with 5 transmembrane domains, appearing as a 120 kDa band due to glycosylation. It is expressed on immature hematopoietic stem cells but not mature blood cells. CD133 is also a marker for neural and embryonic stem cells and is expressed in certain cancers, including leukemias and brain tumors. Low-level expression is observed in kidney, pancreas, placenta, and fetal liver. Mutations in CD133 are linked to retinitis pigmentosa and Stargardt disease. Expression is regulated by multiple tissue-dependent promoters and alternative transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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