Thermo Fisher Scientific HMGB1 Polyclonal Antibody
다양한 응용 분야(WB, IHC, ICC, Flow, ELISA)에 사용 가능한 HMGB1 폴리클로날 항체. 인간, 마우스, 랫트 반응성. Rabbit IgG 기반으로 Protein A 정제. 고순도 액상 형태로 안정적인 -20°C 보관 가능.
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Thermo Fisher Scientific HMGB1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human HMGB1, amino acids 75–170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. It is released by damaged or necrotic cells into the extracellular space, functioning as a proinflammatory cytokine. Mouse HMGB1 consists of 215 amino acids forming three domains: two positively charged DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162) and a negatively charged 30 aa C-terminal acidic tail. Nuclear localization signals are found at residues 28–44 and 180–185. The B box mediates cytokine activity, while the A box interacts with transcription factors. HMGB1 facilitates monocyte migration, cytokine secretion, and T cell–dendritic cell interactions. It signals via RAGE and possibly TLR2/TLR4. HMGB1 is conserved and ubiquitous, acting as a key mediator of inflammation during sterile and infectious responses. Elevated HMGB1 levels are associated with tissue injury, sepsis, arthritis, and ischemia-reperfusion damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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