Thermo Fisher Scientific DGKD Polyclonal Antibody
DGKD 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태로 제공되며, 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 34–88 of Human DGKD |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2718984 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid act as second messengers in cellular signaling cascades. Different DGK isoforms show tissue-specific expression and functional diversity:
- DGK-alpha: Influences cell activation and secretion of exosomes controlling cell death.
- DGK-beta: Abundant in brain regions such as the caudate putamen and olfactory tubercle.
- DGK-gamma: Expressed in various tissues, highest in retina.
- DGK-delta: Predominantly in skeletal muscle.
- DGK-epsilon: Specific for arachidonyl-containing diacylglycerol, mainly in testis.
- DGK-theta: Highly expressed in cerebellum and hippocampus.
- DGK-iota: Expressed in brain and retina, producing multiple transcript variants.
- DGK-eta: Closely related to DGK-delta.
- DGK-zeta: Abundant in brain and muscle.
DGKs contain structural motifs that regulate their activity and are classified into five subtypes based on these features.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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