Thermo Fisher Scientific PIST Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

-

Immunohistochemistry (IHC)

2 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide from near the carboxy terminus of human PIST. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PIST,uniProtId:Q9HD26-1,ncbiNodeId:9606,antigenRange:462,antigenLength:462,antigenImageFileName:PA5-20179_PIST_Q9HD26-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20179_PIST_Q9HD26-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152994

Product Specific Information

A suggested positive control is rat colon lysate.

PA5-20179 can be used with blocking peptide PEP-0294.

Target Information

PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.