Thermo Fisher Scientific Beclin 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10-20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide corresponding to 17 amino acids near the center of human Beclin-1. The immunogen is located between 40-90 amino acids of Beclin-1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Beclin 1,uniProtId:Q14457-1,ncbiNodeId:9606,antigenRange:1,antigenLength:450,antigenImageFileName:PA5-20172_Beclin_1_Q14457-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20172_Beclin_1_Q14457-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11155372

Product Specific Information

Suggested positive controls are mouse heart, mouse brain, or rat heart tissue lysates.

PA5-20172 can be used with blocking peptide PEP-0289.

Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%).

Target Information

Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.