Thermo Fisher Scientific B-Raf Monoclonal Antibody (1H12F1, 1H12G10,1F12F11C9)
인간 BRAF 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체로, WB, IHC, ICC, ELISA에 사용 가능. 비결합형 액상 형태이며 4°C 또는 -20°C에서 보관. 다양한 암 연구 및 MAPK/ERK 신호전달 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H12F1, 1H12G10, 1F12F11C9 |
| Immunogen | Purified recombinant fragment of BRAF expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10987001 |
Product Specific Information
MA5-15317 targets BRAF in indirect ELISA, IHC, and WB applications and shows reactivity with human samples.
The MA5-15317 immunogen is a purified recombinant fragment of BRAF expressed in E. coli.
Target Information
BRAF is involved in transducing mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutations in human cancers and plays a role in postsynaptic responses of hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
Mutations in B-Raf are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma.
A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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