Thermo Fisher Scientific HMGB1 Polyclonal Antibody, DyLight 488
HMGB1 단백질을 표적으로 하는 DyLight 488 형광 표지 폴리클로날 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용 가능. 사람, 생쥐, 토끼 등 여러 종에 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Thermo Fisher Scientific HMGB1 Polyclonal Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:250 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100–200). |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154610 |
Target Information
HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 into the extracellular space, where it acts as a proinflammatory cytokine. Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains: two positively charged DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162) and a negatively charged 30-aa C-terminal acidic tail. Nuclear localization signals are found at residues 28–44 and 180–185.
The cytokine activity resides in box B, while box A interacts with transcription factors. HMGB1 mediates inflammation, monocyte migration, cytokine secretion, and T cell-dendritic cell interaction. It signals through receptors such as RAGE, TLR2, and TLR4. HMGB1 is highly conserved, present in nearly all cell types, and plays a key role in sterile and infection-associated inflammatory responses. Elevated HMGB1 levels are observed during tissue injury, sepsis, arthritis, and ischemia-reperfusion conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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