Thermo Fisher Scientific HMGB1 Polyclonal Antibody, DyLight 650
HMGB1 단백질을 표적으로 하는 DyLight 650 결합 폴리클로날 항체로, Western blot 및 IHC(파라핀) 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 651/673 nm 형광 특성을 가짐. 연구용 전용 제품으로 높은 특이성과 안정성을 제공.
- 카탈로그번호
- PA522720
- 판매단위
- pk
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Thermo Fisher Scientific HMGB1 Polyclonal Antibody, DyLight 650
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:250 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal portion of human HMGB1 (residues 100–200) |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152142 |
Product Specific Information
This antibody will not work in ICC/IF applications.
Target Information
HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 extracellularly, where it acts as a proinflammatory cytokine.
Mouse HMGB1 consists of 215 amino acids with three domains:
- Two positively charged DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
- A negatively charged C-terminal tail (30 aa)
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The B box mediates cytokine activity, while the A box interacts with transcription factors.
HMGB1 functions as an inflammatory mediator promoting monocyte migration and cytokine secretion, and facilitates T cell–dendritic cell interaction.
It signals through RAGE and possibly TLR2/TLR4 receptors.
HMGB1 is highly conserved and ubiquitously expressed, serving as a key mediator of inflammation in sterile and infection-associated responses.
Elevated HMGB1 levels are associated with tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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