Takara Random Primer DNA Labeling Kit Ver. 2

Takara Random Primer DNA Labeling Kit Ver. 2

제품 개요

The Random Primer DNA Labeling Kit Ver. 2 is designed for radioactive labeling of hybridization probes. It enables labeling of DNA with [32P]-alpha-, [35S]-alpha-, or [3H]-alpha-dCTP.
This kit is based on a modified method by Feinberg and Vogelstein (1983, 1984), utilizing random oligonucleotide primers and cloned exonuclease-free E. coli DNA polymerase I (Klenow fragment).
Using primers that are 9-mer or longer and an exonuclease-free enzyme results in higher labeling efficiency and longer probes.
This method overcomes many disadvantages of conventional nick translation procedures and allows probe labeling from very small amounts of DNA (10–20 ng).

주요 특징

• 방사성 동위원소를 이용한 DNA 라벨링 가능
• Exonuclease-free Klenow fragment 사용으로 고효율 표지
• 9-mer 이상의 랜덤 프라이머로 긴 프로브 생성
• 소량 DNA 샘플에서도 높은 감도 확보
• 기존 nick translation 방식 대비 향상된 안정성과 효율

스펙 정보

관련 정보

Find answers to your PCR questions

• Primer design: Successful PCR primer design FAQ
• Optimization: PCR optimization FAQ
• Troubleshooting: PCR troubleshooting FAQ
• Applications and conditions: Application-specific PCR conditions FAQ
• Shipping, storage, and handling: Takara Bio PCR polymerase handling FAQ
• Avoid DNA contamination in PCR: Guide to prevent common PCR contamination problems

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