Takara Random Primer DNA Labeling Kit Ver. 2

The Random Primer DNA Labeling Kit Ver. 2 is designed for radioactive labeling for hybridization probes. Probe labeling can label DNA with [32P]-alpha-, [35S]-alpha-, or [3H]-alpha-dCTP. This random primer DNA labeling kit is based on a modified method by Feinberg and Vogelstein (Feinberg and Vogelstein 1983; Feinberg and Vogelstein 1984), and utilizes random oligonucleotide primers and cloned exonuclease-free E.coli DNA polymerase I, Klenow fragment. Using primers that are 9-mer and longer and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This probe labeling method overcomes many of the disadvantages of conventional nick translation procedures while producing hybridization probes from very small amounts of DNA (10 ng to 20 ng).

Find answers to your PCR questions

[Primer design](/learning-centers/pcr/faq/primer-design Primer design)

Frequently asked questions about primer design for successful PCR.

[Optimization](/learning-centers/pcr/faq/optimization Optimization)

Frequently asked questions about PCR optimization.

[Troubleshooting](/learning-centers/pcr/faq/troubleshooting Troubleshooting)

Frequently asked questions about troubleshooting your PCR problems.

[Applications and conditions](/learning-centers/pcr/faq/applications-and-conditions Applications and conditions)

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

[Shipping, storage, and handling](/learning-centers/pcr/faq/shipping-storage-and-handling Shipping, storage, and handling)

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

[Avoid DNA contamination in PCR](/about/bioview-blog/tips-and-troubleshooting/avoid-dna-contamination-in-pcr Avoid DNA contamination in PCR)

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.