Thermo Fisher Scientific Phospho-CaMKII alpha/beta/delta (Thr305) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fruit fly

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of threonine 305 (I-L-T(p)-T-M) derived from Human CaMK2-alpha-beta-delta if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CaMKII alpha/beta/delta,uniProtId:Q9UQM7-1,ncbiNodeId:9606,antigenRange:303-307,antigenLength:478,antigenImageFileName:PA5-37832_CaMKII_alphabetadelta_Q9UQM7-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37832_CaMKII_alphabetadelta_Q9UQM7-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:CaMKII alpha/beta/delta,uniProtId:Q13554-1,ncbiNodeId:9606,antigenRange:303-307|304-308,antigenLength:666,antigenImageFileName:PA5-37832_CaMKII_alphabetadelta_Q13554-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37832_CaMKII_alphabetadelta_Q13554-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:CaMKII alpha/beta/delta,uniProtId:Q13557-1,ncbiNodeId:9606,antigenRange:303-307|304-308,antigenLength:499,antigenImageFileName:PA5-37832_CaMKII_alphabetadelta_Q13557-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37832_CaMKII_alphabetadelta_Q13557-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554440

Product Specific Information

A suggested positive control for Western blot is NIH-3T3 cells; suggested positive control for IHC is human brain tissue.

Target Information

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated alpha, beta, gamma and delta, which may or may not be co-expressed in the same tissue type. CaMKII alpha is autophosphorylated on Thr 286 upon the binding of the Ca2+/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca2+/CaM trapping, which is thought to be involved in the synaptic encoding of information.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.