Thermo Fisher Scientific Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioM3/38 (M3/38)

Immunogen

Rec. Human Galectin-3

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• eFluor 660
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10597892

Product Specific Information

Description: The Anti-human Galectin-3 (M3/38) antibody recognizes an epitope in the N-terminal half of lectin. Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up- regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

Applications Tested: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blotting.

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.