Thermo Fisher Scientific TNF beta Monoclonal Antibody (LTX-22), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LTX-22

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10597128

Product Specific Information

Description: BMS1046 (LTX-22) is reportded for ELISA and ICH applications.

Applications Tested: ELISA, Immunohistochemistry (Frozen).

Source: Plant expressed 164 amino acids (Cys32-Thr194) with 16 amino acid Histidine tag. accession NP_002000.

Bioactivity: The ED50 of this protein, as measured by proliferation assay of 4MBr5 monkey epthelial cells, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg.

Endotoxin: Less than 0.005 ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE.

Molecular Weight: 21.2 kDa but with glycosylation the apparent molecular weights are 26 and 28 kDa.

Target Information

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.