Thermo Fisher Scientific Histone H3 Recombinant Rabbit Monoclonal Antibody (27F2)
Histone H3 단백질을 표적으로 하는 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 친화 크로마토그래피 정제. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:30–1:200 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 27F2 |
| Immunogen | A synthesized peptide (3–55aa) of Histone H3 (UniProt ID: P68431-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898198 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with other core histone proteins, binds to DNA to form the nucleosome structure. Histones play a key role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression.
Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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