
Thermo Fisher Scientific Alpaca anti-Mouse IgG1 Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor 568
Alpaca 유래 재조합 단일클론 나노항체로, Mouse IgG1을 특이적으로 인식. Alexa Fluor 568 결합으로 형광 검출에 최적. Western blot, ICC/IF, Flow cytometry 등 다양한 응용 가능. 비교종 반응성 낮고, 빠른 워크플로우 제공.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | Assay-dependent |
| Dot blot (DB) | Assay-dependent |
| Immunomicroscopy (IM) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Alpaca / IgG1 |
| Expression System | E. coli |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | CTK0103 / CTK0104 |
| Immunogen | Purified mouse IgG1 |
| Conjugate | Alexa Fluor™ 568 |
| Excitation/Emission Max | 579 / 603 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2868377 |
| Target | IgG1 |
| Antibody Form | VHH |
Product Specific Information
- Biclonal nanobodies specific for mouse IgG1 Fc.
- CTK0103: KD = 0.13 nM
- CTK0104: KD = 0.63 nM
- Non Cross-Reactivity: Goat, guinea pig, rabbit, rat, sheep, human, macaque serum, and mouse IgG2a, IgG2b, IgG2c, IgG3.
- Rapid workflow: Simultaneous incubation of mouse IgG1 primary antibody and Alpaca anti-mouse IgG1 Nano (VHH) recombinant secondary antibody.
- Offered through a partnership with ChromoTek.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
These are useful in detection, sorting, or purification of specific targets.
Secondary antibodies provide versatility across detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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