Thermo Fisher Scientific Alpaca anti-Mouse IgG1 Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor 488

Applications

Tested Dilution

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:500

Flow Cytometry (Flow)

Assay-dependent

Dot blot (DB)

Assay-dependent

Immunomicroscopy (IM)

1:500

Product Specifications

Host/Isotype

Alpaca / IgG1

Expression System

E. coli

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

CTK0103/CTK0104

Immunogen

Purified mouse IgG1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2868375

Target

IgG1

Antibody Form

VHH

Product Specific Information

Biclonal nanobodies specific for mouse IgG1 Fc. CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM

Non Cross-Reactivity includes: Goat, guinea pig, rabbit, rat, sheep, human, and macaque serum and to mouse IgG2a, IgG2b, IgG2c, and IgG3.

Rapid workflow: Simultaneous incubation of mouse IgG1 primary antibody and Alpaca anti-mouse IgG1 Nano (VHH) recombinant secondary antibody.

Offered through a partnership with ChromoTek.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.