Thermo Fisher Scientific Alpaca anti-Rabbit IgG Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor 488

Applications

Tested Dilution

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:1,000

Dot blot (DB)

Assay-dependent

Immunomicroscopy (IM)

1:1,000

Product Specifications

Host/Isotype

Alpaca / IgG

Expression System

E. coli

Class

Recombinant Monoclonal

Type

Secondary Antibody

Clone

CTK0101/CTK0102

Immunogen

Purified rabbit IgG

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2868369

Target

IgG

Antibody Form

VHH

Product Specific Information

Biclonal nanobodies specific for rabbit IgG Fab and Fc. CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM

Non Cross-Reactivity includes: Mouse, rat, sheep, goat, and guinea pig serum. Cross-reactivity to: Human and macaque serum

Rapid workflow: Simultaneous incubation of rabbit IgG primary antibody and Alpaca anti-rabbit IgG Nano (VHH) recombinant secondary antibody.

Offered through a partnership with ChromoTek.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.