Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), eFluor 506, eBioscience
인간 CD19를 인식하는 HIB19 단클론 항체로, eFluor 506 형광체로 결합되어 Flow Cytometry에 최적화됨. B세포 발현 분석에 사용되며, 405 nm 레이저로 여기, 506 nm에서 방출. 액상 형태로 제공되며 4°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 26 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 506, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIB19 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637383 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Blue / Yellow / Red series
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series
- NovaFluor series
- Functional Grade
- Custom conjugation available
Product Specific Information
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the B cell receptor (BCR). Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation.
The SJ25C1 and HIB19 antibodies recognize overlapping epitopes.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Use 5 µL (0.5 µg) per 100 µL test volume, with 10⁵–10⁸ cells/test recommended.
Fluorochrome Information:
eFluor® 506 is excited by the 405 nm violet laser and emits at 506 nm. Recommended detection filter: 510/20 band pass or equivalent.
Excitation: 405 nm
Emission: 506 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on nearly all peripheral B cells and certain myeloid leukemia cells. It is a key regulator of B cell activation and proliferation, functioning as a signal-amplifying co-receptor with the BCR. Mutations in CD19 cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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