DMEM, high glucose, no glutamine, no phenol red

DMEM, high glucose, no glutamine, no phenol red

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
With
• High Glucose

Without

• L-glutamine
• Phenol Red
• Sodium Pyruvate
• HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system

DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

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주문 전 상세 내용 다시 한번 확인 부탁드립니다.

사양

세포주	HeLa, 293, Cos-7, and PC-12
세포 유형	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
분류	Animal Origin-free
농도	1 X
형태	액체
제품 유형	DMEM (Dulbecco's Modified Eagle Medium)
Serum Level	Standard Serum Supplementation
유통 기한	12 Months From Date of Manufacture
멸균	Sterile-filtered
첨가제 포함	High Glucose
첨가제 없음	No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
Glucose 농도	4500 mg/L
그린 기능	Sustainable packaging
제조 품질	cGMP-compliant under the ISO 13485 standard
제품라인	Gibco™

구성 및 보관

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

배지 조성

31053 - DMEM, high glucose, no glutamine, no phenol red