Thermo Fisher Scientific PKN1 Polyclonal Antibody
Western blot 및 ELISA에 적합한 PKN1 폴리클로날 항체. 인간 및 생쥐 반응성, Rabbit IgG 유래. 친화 크로마토그래피 정제, PBS/glycerol buffer에 보관. -20°C에서 보관하며 반복 동결-해동 회피 필요.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–300 of human PKN1 (NP_002732.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.53 mg/mL |
| Purification | Affinity Chromatography |
| Storage buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2804162 |
Product Specific Information
Immunogen sequence:
MASDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLRREIRKEL KLKEGAENLR RATTDLGRSL GPVELLLRGS SRRLDLLHQQ LQELHAHVVL PDPAATHDGP QSPGAGGPTC SATNLSRVAG LEKQLAIELK VKQGAENMIQ TYSNGSTKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA GQLENQAAPD DTQGSPDLGA VELRIEELRH HFRVEHAVAE GAKNVLRLLS AAKAPDRKAV SEAQEKLTES NQKLGLLREA LERRLGELPA DHPKGRLLRE ELAAASSAAF
Positive Samples:
U-251MG, A-549, 293T, HeLa, HepG2, HT-1080, Mouse kidney, Mouse spleen
Cellular Location:
Cell membrane, Cleavage furrow, Cytoplasm, Endosome, Midbody, Nucleus, Peripheral membrane protein
Target Information
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. It can also be activated by phospholipids. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) phosphorylates this kinase, mediating insulin signals to the actin cytoskeleton. Proteolytic activation by caspase-3 or related proteases during apoptosis suggests its role in apoptotic signal transduction. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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