Thermo Fisher Scientific Ly-49H Monoclonal Antibody (3D10), eBioscience
Ly-49H 단클론 항체(3D10)는 자연살해세포(NK cell)에서 발현되는 활성 수용체를 인식합니다. Flow Cytometry 및 기능 분석에 적합하며, Mouse IgG1, kappa 형식의 비결합 항체입니다. 0.5 mg/mL 농도, PBS 완충액에 보관하며 4°C에서 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.25 µg/test | View 12 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay (Functional) | Assay-Dependent | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865167 |
Available Conjugated Formats
Product Specific Information
The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer (NK) cells. Ly-49H, similar to Ly-49D, lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine residue in the transmembrane domain, suggesting its role as an activation receptor. Ly-49H is expressed in a subset of NK cells but not in NKT (NK1.1⁺CD3⁺) cells.
Natural killer cells can express several Ly-49 proteins. Preferential staining of Ly-49H along with Ly-49D has been observed. Expression confirmed in C57BL/6 and NWNA mice but not in Balb/c or DBA/2 strains. No cross-reactivity to Ly-49A, C, D, or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.
Applications Reported:
This 3D10 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of C57Bl/6 splenocytes. Recommended ≤0.5 µg per test (defined as staining a cell sample in 100 µL final volume). Optimal performance should be determined empirically.
Optical Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Like Ly49D, it lacks the ITAM motif and contains an arginine in the transmembrane domain, functioning as an activation receptor. Expressed in NK cells but not NKT (NK1.1/CD3⁺) cells. NK cells can express several Ly49 proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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