Thermo Fisher Scientific FEN1 Monoclonal Antibody (OTI2B2), TrueMAB
Human FEN1 단백질(aa117-380)에 특이적인 Mouse IgG1 단클론 항체로, Western blot에 최적화됨. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. DNA 복제 및 수선 연구용으로 적합.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 117–380 of human FEN1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein functioning as an endonuclease that cleaves branched DNA structures containing single-stranded 5′ flaps or as an exonuclease on DNA nicks and gaps.
Its activities are stimulated by association with PCNA (Proliferating Cell Nuclear Antigen) in the nucleus.
FEN-1 plays an essential role in DNA replication and repair, and is required in cell-free DNA replication systems.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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