Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
DyLight 550 형광이 결합된 Donkey anti-Goat IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 실험에 사용 가능. 교차흡착 처리로 비특이적 반응 최소화. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공.
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Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Goat IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556667 |
| Target | IgG |
| Cross Adsorption | Against chicken, human, mouse, pig, rabbit, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to chicken, human, mouse, pig, rabbit, and rat.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling use of various detection systems (e.g., HRP, AP, fluorescence). They provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, Biotin, DSB-X Biotin
- DyLight 350, 488, 594, 650, 680, 755, 800
- FITC, HRP, TRITC
- Custom conjugation available upon request
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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