Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800
DyLight 800 형광 표지된 Rabbit anti-Goat IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 다양한 종에 대한 교차 반응 최소화. 고순도 항원 친화 크로마토그래피 정제. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Goat IgG-heavy and light chain |
| Conjugate | DyLight™ 800 |
| Excitation/Emission Max | 789 / 794 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556664 |
| Target | IgG |
| Cross Adsorption | Against chicken, horse, human, mouse, pig, and rat |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488, 546, 555, 568, 594, 633, 647, 680
- AP, Biotin, Biotin-XX
- DyLight 488, 550, 594, 650, 680
- FITC, HRP, TRITC
- Custom conjugation available upon request
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to chicken, horse, human, mouse, pig, and rat.
For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and antibody incubation. BSA is not recommended.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins.
They are useful for detection, sorting, or purification of goat IgG targets.
Secondary antibodies increase versatility and sensitivity by allowing multiple detection systems (HRP, AP, fluorescence) and signal amplification.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and optional modifications such as immunoaffinity chromatography, fragmentation, or label conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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